Standardization of an Herbal Formulation (DIA-2) Containing Allium sativum and Lagerstroemia speciosa extracts using HPTLC-UV densitometry
نویسنده
چکیده
The bulbs of Allium sativum (ASE) and leaves of Lagerstroemia speciosa (LSE) are traditionally used to treat a variety of health disorders in the southern part of India. DIA-2 is a herbal formulation developed to contain fixed ratio (1:1 w/w) of ASE and LSE. In the present study, the phytochemical analysis of ASE and LSE was carried out following standard protocols. Also, a simple, rapid and reproducible high performance thin-layer chromatographic (HPTLC) fingerprint of DIA-2 and its ingredients was developed. A characteristic HPTLC fingerprint has been generated for ASE, LSE and DIA-2 using appropriate mobile phase and precoated plates with silica gel 60 F254 as stationary phase. Densitometric scanning of LSE and DIA-2 were performed at 366 nm and ASE at 515 nm after derivatization with 0.2% ninhydrin (in acetone). The HPTLC fingerprint of DIA-2 was found to be reproducible and revealed nine peaks at the selected wavelength. This study provides a simple, rapid, reproducible and economic chromatographic technique for qualitative standardisation of DIA-2 and its ingredients.
منابع مشابه
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